Journal: Stem Cell Research & Therapy

Article Title: Activation of the G-protein coupled estrogen receptor 1 (GPER1) reduces transient receptor potential vanilloid 1 (TRPV1) activity and human iPSC-derived nociceptive neuron firing

doi: 10.1186/s13287-026-05174-3

Figure Lengend Snippet: Reprogramming of adult human dermal fibroblasts (HDFa) into induced pluripotent stem cell (iPSC) line BO-VC1. Reprogramming was performed using the Epi5 ™ Episomal iPSC Reprogramming Kit, enabling the generation of ( A ) transgene- and virus-free iPSC line BO-VC1. Successful generation of fibroblast-derived iPSCs was validated by immunocytochemical staining for the pluripotency markers ( B ) SOX2, C TRA 1–60, D OCT4, SSEA4 and E NANOG. Quantification of the generated iPSCs via flow cytometry revealed F 99.31% SSEA4/TRA1-60, G 97.43% SOX2/TRA1-60 and H 98.60% OCT3/4/TRA1-60 positive cells. I Quantification of the transcript levels of the stem cell markers NANOG , OCT4 , REX1 and SOX2 revealed significantly higher mRNA levels in iPSC line BO-VC1 compared to the HDFa control ( n = 3 different passages) Moreover, generated iPSCs were functionally validated by directed differentiation into all three germ layers. Subsequent immunocytochemical staining of the iPSCs before and after differentiation confirmed the expression of ( J–M ) meso-, N–Q endo- and R–U ectodermal lineage markers only in the respective differentiations. Scale bars: 50 μm. Data were tested for normal distribution using Shapiro-Wilk test. Means ± SEM (standard error of the mean) were statistically analyzed by a Kruskal-Wallis test with Dunn’s multiple comparisons test. n = 3 (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001)

Article Snippet: Subsequently, cells were incubated in inside fix solution of the Inside Stain Kit (#130-090-477, Miltenyi Biotec, Bergisch Gladbach, Germany) for 20 min at RT and centrifuged at 200xg for 5 min. After washing in 500 μl PEB buffer and centrifugation at 200xg cells were stained with the nuclear stem cell markers OCT3/4 (1:50, #130-117-821, Miltenyi Biotec, Bergisch Gladbach, Germany) and SOX2 (1:50, #130-120-790, Miltenyi Biotec, Bergisch Gladbach, Germany) in inside perm solution for 10 min at RT.

Techniques: Virus, Derivative Assay, Staining, Generated, Flow Cytometry, Control, Expressing

Journal: Frontiers in Neuroscience

Article Title: A pH-responsive and self-healing CEC/PEG-(CHO) 2 hydrogel for controlled neuropeptide S delivery and endogenous neural repair after spinal cord injury

doi: 10.3389/fnins.2026.1933351

Figure Lengend Snippet: Phenotypic characterization of mouse NSPCs and the Pro-neurogenic effects of NPS loaded CEC/PEG-(CHO) 2 hydrogel scaffold In Vitro. (a) After 7 days in proliferation medium, isolated NSPCs formed neurospheres. Scale bar = 100 μm. (b–d) Neurospheres were positive for the NSPC markers Nestin, SOX2, and PAX6. Scale bar = 100 μm. (e–g) Quantification of Nestin + , SOX2 + , and PAX6 + cells. (h) Schematic diagram of NSPC isolation. (i) Schematic of the NSPC differentiation assay. (j) After 7 days of differentiation, immature neurons were immunostained for Tuj-1. Scale bar = 50 μm. (k) After 14 days, mature neurons were immunostained for MAP2. Scale bar = 50 μm. (l,m) Quantification of Tuj-1 + immature neurons (l) and MAP2 + mature neurons (m) . ** P < 0.01, *** P < 0.001. (n,o) qPCR analysis of transcription factors associated with neuronal differentiation at 7 (n) and 14 (o) days. * P < 0.05, ** P < 0.01, *** P < 0.001. (p) Schematic of the NSPC axon growth assay. (q) After 7 days in proliferation culture, axons were immunostained for GAP43. Scale bar = 200 μm. (r) Quantification of axon length. (s) qPCR analysis of transcription factors regulating axon growth. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The following primary antibodies were used: Nestin (rabbit, Proteintech, 1:400; NSPCs), SOX2 (rabbit, Proteintech, 1:400; NSPCs), PAX6 (rabbit, Proteintech, 1:400; NSPCs), TUJ1 (mouse, Proteintech, 1:400; immature neurons), MAP2 (mouse, Abcam, 1:400; mature neurons), GFAP (rat, Thermo Fisher, 1:400; astrocytes), GAP43 (rabbit, Proteintech, 1:400; axons), MMP-9 (rabbit, Abclonal, 1:400; secreted MMP-9), TNF-α (mouse, Proteintech, 1:400; pro-inflammatory cytokines), IL-10 (mouse, Proteintech, 1:400; anti-inflammatory cytokines), CD68 (rat, Abcam, 1:400; activated immune cells), CSPGs (mouse, Cell Signaling Technology, 1:400; glial scar), synaptophysin (SYP, rabbit, Proteintech, 1:400; presynaptic membranes), and cAMP (rabbit, Proteintech, 1:400; intracellular second messenger).

Techniques: In Vitro, Isolation, Differentiation Assay, Analysis, Growth Assay

Journal: Stem cell research

Article Title: Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients with TTN mutations

doi: 10.1016/j.scr.2026.104065

Figure Lengend Snippet: Reagents details.

Article Snippet: Undifferentiated hPSC state marker , Mouse IgG1κ Anti-Sox2 , 1:200 , Santa Cruz Biotechnology Cat #sc-365823 , AB_10842165.

Techniques: Immunocytochemistry, Marker